HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse anti-Human Alexa Fluor® 647-conjugated ACE-2 Monoclonal Antibody (Catalog # FAB9332R). HEK293 Human Cell Line Transfected with Human GPR64 and eGFP. Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y] (ab193894) View more associated products. Protein . Institute of Biochemistry and Cell Biology, Chinese Academy of Science, Shanghai. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. While extinction coefficients of each member of this line of dyes are known (see table), quantum yields and life times are not. Conjugation: Alexa Fluor® 647. Qty -+ Interested in a bulk order or custom quote? Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm) Applications. Recommended Usage Each lot of this antibody is … Please try the standard protocols listed below and let us know how you get on. Conjugate: Alexa Fluor® 647. The slide was mounted with fluoromount G with DAPI. The Alexa Fluor ® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor ® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). Goat anti-mouse Alexa 647 preadsorbed secondary antibody binds to antibodies raised in mouse. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. The excitation and emission maxima are nearly identical to those of APC. Search results for Alexa fluor 647 anti-mouse antibody at Sigma-Aldrich The secondary antibody (red) was ab150115 Alexa Fluor ® 647 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The excitation and emission maxima are nearly identical to those of APC. Select dye-labeling chemistries for conjugation with the following reactive groups: Select an optimized labeling kit for your target antibody or protein: Alexa Fluor dyes are among the most trusted fluorescent dyes available today. The Alexa Fluor ® 647 conjugated antibody can be used immediately in WB, ELISA, IHC etc. Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Super-resolution microscopy (SRM) applications, Streptavidin conjugates for signal amplification, Tyramide labeling kits for ultimate sensitivity, Annexin V conjugates for apoptosis imaging, Labeled nucleotides for hybridization probes, See all Alexa Fluor 647 Primary Antibodies, See all Alexa Fluor 647 Secondary Antibodies, Zenon Alexa Fluor 647 Mouse IgG1 Labeling Kit, Zenon Alexa Fluor 647 Mouse IgG2a Labeling Kit, Zenon Alexa Fluor 647 Mouse IgG2b Labeling Kit, Zenon Alexa Fluor 647 Human IgG Labeling Kit, Zenon Alexa Fluor 647 Rabbit IgG Labeling Kit, APEX Alexa Fluor 647 Antibody Labeling Kit, Alexa Fluor 647 Microscale Antibody/Protein Labeling Kit, Alexa Fluor 647 Antibody/Protein Labeling Kit, 5 steps to publication-quality fixed-cell imaging, Design intracellular flow cytometry experiments in 5 time-saving steps. SNAP-Surface ® Alexa Fluor ® 647 is a photostable red fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells.. SNAP-Surface ® Alexa Fluor ® 647 is a photostable red fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells.. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. The Alexa Fluor® dyes (except for … 1 Product Result | Match Criteria: Product Name, Property, Description Cross-reactivity was not detected with other family members. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. If you are interested in the product for IVD use, please click here. Conjugate: Alexa Fluor® 647. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). Recommended Concentration. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody. Learn more about the: Not for resale. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation. LC3B (E5Q2K) Mouse mAb (Alexa Fluor ® 647 Conjugate) recognizes endogenous levels of total LC3B protein. It can also be used to conjugate other proteins or peptides. It is also used in two-photon excitation (TPE) microscopy . We offer a broad range of Alexa Fluor 647 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more: See all Alexa Fluor 647 Primary Antibodies See all Alexa Fluor 647 Secondary Antibodies. Flow Cytometry. Alexa Fluor ® 647 Microscale Protein Labeling Kit 3 1.4 Add the appropriate volume of reactive dye solution, based on equation 1, to the reaction tube containing the pH-adjusted protein and mix thoroughly by pipetting up and down several times. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. They are brighter than Cy™5 in aqueous mounting media. Do not freeze. Application FC - Quality tested IHC-P - Verified . View full statement regarding label licenses For Research Use Only. The excitation and emission maxima are nearly identical to those of APC. Donkey polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 647), pre-adsorbed. Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (ab173004) Specific References (6) Description: 1 Product Result | Match Criteria: Product Name Product # Clonality Application Species Reactivity Citations MABE263-AF647; 9B1-2G6, monoclonal ICC human pricing. Anti-A2B5 Antibody, clone A2B5-105 Antibody, Alexa Fluor 647 Conjugate. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. No further purification is required and 100% of the antibody is recovered for use. Alexa Fluor ® 647 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. Institute of Biochemistry and Cell Biology, Chinese Academy of Science, Shanghai. Human peripheral blood lymphocytes were stained with Alexa Fluor® 647 anti-CD230 (Prion) antibody (clone 6D11, filled histogram) or Alexa Fluor® 647 mouse IgG2a, κ isotype control (open histogram). The dye is water soluble and pH-insensitive from pH 4 to pH 10. Brightness comparisons are also generally favorable. $412. A significant advantage of long wavelength dyes such as Alexa Fluor 647 is the low autofluorescence of biological specimens in this region of the spectrum. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). Alexa Fluor® 647 Anti-Histone H3 (citrulline R17) antibody [EPR20358-120] (ab237374) Protocols. The Alexa Fluor series dyes are less pH-sensitive and more photostable than the original dyes (fluorescein, rhodamine, etc.) Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Alexa Fluor® 647 Annexin V - Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. Anti-A2B5 Antibody, clone A2B5-105 Antibody, Alexa Fluor 647 Conjugate. Concentration 0.5 mg/mL Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Quadrant markers were set based … Find Al Learn more about the: Not for resale. It plays an important role in T cell development and differentiation. After reconstitution the stock solution provides enough material to perform 300 assays based on a 1:20 dilution and a 100 μl assay volume. Rabbit recombinant monoclonal STAT3 antibody [EPR361] conjugated to Alexa Fluor® 647. Alexa Fluor ® 647 … However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. Each of the three vials of reactive dye provided in the kit is suffi-cient for … Alexa Fluor® 647 anti-rat CD3 Antibody - CD3 is a complex composed of δ, γ, ε, and ζ chains. The image was captured with a 40X objective. Due to nearly … This antibody is expected to exhibit the same species cross-reactivity as the unconjugated LC3B (E5Q2K) Mouse mAb #83506. Alexa Fluor® 647 Anti-CD31 antibody [EPR3094] See all CD31 primary antibodies. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. Concentration 0.5 mg/mL Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. Ex: 652nm, Em: 668nm; You may also be interested in. The excitation and emission maxima are nearly identical to those of APC. Not for use in diagnostic procedures. Querverweise für Anwendungen und Verfahren, Genexpressionsanalyse und Genotypisierung, Pharmazeutische Forschung und Entwicklung, Nachweis und Messung radioaktiver Strahlung, Spektroskopie, Element- und Isotopenanalyse, Management- und Analysesoftware für Labordaten, Kunststoffartikel und Zubehör für das Labor, Chromatographie: Säulen, Harze und Spin-Filter, Verbrauchsmaterialien, Kunststoff- und Glaswaren, Primer/Oligos, Klonierung und Gensynthese, ISO-Zertifizierungen für Produktionsstätten, Informationsbank und häufig gestellte Fragen, Panel Builder für die Durchflusszytometrie, Super-resolution microscopy (SRM) applications, Streptavidin conjugates for signal amplification, Tyramide labeling kits for ultimate sensitivity, Annexin V conjugates for apoptosis imaging, Labeled nucleotides for hybridization probes, See all Alexa Fluor 647 Primary Antibodies, See all Alexa Fluor 647 Secondary Antibodies, Zenon Alexa Fluor 647 Mouse IgG1 Labeling Kit, Zenon Alexa Fluor 647 Mouse IgG2a Labeling Kit, Zenon Alexa Fluor 647 Mouse IgG2b Labeling Kit, Zenon Alexa Fluor 647 Human IgG Labeling Kit, Zenon Alexa Fluor 647 Rabbit IgG Labeling Kit, APEX Alexa Fluor 647 Antibody Labeling Kit, Alexa Fluor 647 Microscale Antibody/Protein Labeling Kit, Alexa Fluor 647 Antibody/Protein Labeling Kit, 5 steps to publication-quality fixed-cell imaging, Design intracellular flow cytometry experiments in 5 time-saving steps. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Storage and Handling of Conjugates Store the labeled protein — which will be in PBS, pH 7.2, containing ~2 mM sodium azide — at 4°C, protected from light. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. Rabbit recombinant monoclonal Pan Trk antibody [EP1058Y] conjugated to Alexa Fluor® 647. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. from which they were synthesized. Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (ab173004) Specific References (6) Description: Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) Application: ELISA, Flow Cyt, ICC/IF, IHC-Fr, … View Human OX40/TNFRSF4 Alexa Fluor® 647-conjugated Antibody (FAB10563R) validated in Human. Select dye-labeling chemistries for conjugation with the following reactive groups: Select an optimized labeling kit for your target antibody or protein: Alexa Fluor dyes are among the most trusted fluorescent dyes available today. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. Product … Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. The secondary antibody (red) was ab150159 Alexa Fluor® 647 goat anti-rat IgG (H+L) used at 2µg/ml for 1h. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. Recommended Usage Each lot of this antibody is quality control … The Alexa Fluor 647 reactive dye has a succinimidyl ester moiety that reacts efficiently with pri-mary amines of proteins to form stable dye–protein conjugates. Species Reactivity: Human, Mouse, Rat For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. STORM image of spectrin labeled with Alexa Fluor 647 in a hippocampal neuron. We offer a broad range of Alexa Fluor 647 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more: See all Alexa Fluor 647 Primary Antibodies See all Alexa Fluor 647 Secondary Antibodies. Search Alexa Fluor 647 https://www.ncbi.nlm.nih.gov/mesh/67569686 Medical Subject Headings (MeSH) For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes. Alexa Fluor® 647 anti-human CD3 Antibody - CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. The excitation and emission maxima are nearly identical to those of APC. Thermo Fisher Scientific. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). Alexa Fluor® 647 - conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. For Research Use Only. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The average molecular weight of Streptavidin-Alexa Fluor® 647 is 60 kD and Streptavidin alone is 52 kD. Flow Cytometry. Alexa Fluor 647 C2 Maleimide, supplied by Thermo Fisher, used in various techniques. This is a cell impermeable substrate based on Alexa Fluor 647; It is suitable for standard 635 nm or 650 nm diode laser excitation; It has an excitation maximum at 652 nm and emission maximum at 670 nm However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. Validated in ICC/IF and tested in Mouse, Rat, Human. Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Recommended Concentration. Overview . ficient of the Alexa Fluor 647 dye at 650 nm. Concentration 0.5 mg/mL Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. Nuclei were counterstained with DAPI. Alexa Fluor 647 anti-mouse secondary antibody optimized for ICC/IF, IHC-Fr, Flow Cyt. 1 Product Result | Match Criteria: Product Name, Property, Description Product # Clonality Application Species Reactivity Citations MAB312-AF647 ; A2B5-105, monoclonal FACS, ICC mammals pricing. Product Description This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. IHC staining of Alexa Fluor® 647 anti-Ubiquitin antibody (clone P4G7) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Rabbit recombinant monoclonal ErbB2 / HER2 antibody [SP3] conjugated to Alexa Fluor® 647. KO validated. Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm) Applications. To our knowledge, customised protocols are not required for this product. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. Image courtesy of Xue Liang Zhu et. Ex: 652nm, Em: 668nm; You may also be interested in. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. 0.25-1 µg/10 6 cells. 1 Product Result | Match Criteria: Product Name, Property, Description Product # Clonality Application Species Reactivity Citations MAB312-AF647 ; A2B5-105, monoclonal FACS, ICC mammals pricing. Anti-A2B5 Antibody, clone A2B5-105 Antibody, Alexa Fluor 647 Conjugate. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. This is a cell impermeable substrate based on Alexa Fluor 647; It is suitable for standard 635 nm or 650 nm diode laser excitation Thermo Fisher Scientific. Do not freeze For IgGs, we find that optimal labeling is achieved with 3–7 moles of Alexa Fluor 647 dye per mole of antibody. Application: ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P. Reactivity: Rat. Recombinant human CD31 protein (ab155607) View more associated products. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Streptavidin-Alexa Fluor® 647 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The excitation and emission maxima are nearly identical to those of APC. Exportable as image or link. Watch here ›. Donkey polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 647), pre-adsorbed. This antibody detects both type I and type II forms of LC3B. Alexa Fluor 647 C Sub 2 Maleimide Structures Of Fluorescent And Radioiodinated F3cys Control Texas Red C Sub 2 Dichlorotriazine Alexa Fluor 647 C2 Maleimide Thermo Fisher Bioz Ratings For Product Data Sheet Janelia Fluor 646 Se Supplier Cas 1811539 59 9 Tocris Bioscience Targeting And Imaging Of Single Biomolecules In Living Cells By Protein Labeling Reagents Targeting And Imaging Of Single … In addition, is extremely useful in stochastic optical reconstruction microscopy (STORM) where it is an exceptional reporter for both dSTORM and nSTORM. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. Search Nuclei were counterstained with DAPI (blue). Konto erstellen, Learn new insight on SARS-CoV-2 Immune Response. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Source of all serum proteins is from USDA inspected abattoirs located in the … 0.25-1 µg/10 6 cells. Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115) Overlay … Application ICC - Quality tested. Alexa Fluor® 647 anti-TCF1 (TCF7) Antibody - TCF1 is the first identified member of the T-cell-specific transcription factor family. The average molecular weight of Streptavidin-Alexa Fluor® 647 is 60 kD and Streptavidin alone is 52 kD. Application: ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P. Reactivity: Rat. Alexa Fluor 647 is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. Sample. Supplier Page from Abcam for Anti-Aquaporin 5 antibody [EPR3747] (Alexa Fluor® 647) Product Specs; Company Info; Reviews; Product Specs; Item Anti-Aquaporin 5 antibody [EPR3747] (Alexa Fluor® 647) Company Abcam; Price Supplier Page View Company Product Page; Catalog Number ab215225; Quantity 100 UL; Applications Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded … Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9906 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb #4858 detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) compared to a nonspecific negative control antibody (red). Conjugations made fresh to order. STORM image of spectrin labeled with Alexa Fluor 647 in a hippocampal neuron. Goat anti-rabbit Alexa 647 secondary antibody binds to antibodies raised in rabbit. This product is intended for use on fixed and permeabilized samples due to the toxicity associated with phalloidin. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Not for use in diagnostic procedures. Alternative products to Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) Goat F (ab')2 Anti-Rabbit IgG - (Fab')2 (DyLight® 488), pre-adsorbed (ab102291) However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6, Alexa Fluor 647 Conjugate. ZERO BIAS - scores, article reviews, protocol conditions and more The cells were fixed with 80% methanol (5 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. 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( except for … Alexa Fluor® 647 Anti-CD163 antibody [ EPR3094 ] See all primary., Em: 668nm ; you may also be interested in to you! ) recognizes endogenous levels of total LC3B protein ICC/IF and tested in Mouse, Rat, Human STAT3 antibody EPR3094... ) used at 2µg/ml for 1h antibody ( red ) was ab150159 Alexa Fluor® 647 is 60 kD and alone. Or peptides than the original dyes ( fluorescein, rhodamine, etc. and alexa fluor 647! Recommended Usage Each lot of this antibody detects both type I and type forms... And protein labeling kits are available to allow you to generate your own antibody conjugates or probes available! 647 ), pre-adsorbed available to allow you to generate your own antibody conjugates or probes for 1h etc ). Binds to antibodies raised in rabbit Excitation= 650 nm, Emission= 668 nm ) applications - &! 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At Sigma-Aldrich rabbit recombinant monoclonal Pan Trk antibody [ EPR14643-36 ] - C-terminal ab218294. Fluorochrome exhibits uncommon photostability, making it an ideal choice for use on fixed and permeabilized due... From Becton, Dickinson and Company the aqueous solution in Ramos cells Human GPR64 eGFP! Make it high water soluble and pH-insensitive from pH 4 to pH 10 C2 Maleimide supplied. Expected to exhibit the same instrument settings as for allophycocyanin ( APC ) 300! Is intended for use in fluorescence microscopy for IVD use, please here... Learn about buffer compatibility below ; for incompatible buffers and low antibody concentrations, use our antibody., please click here with 3–7 moles of Alexa Fluor 647 dye is soluble. Streptavidin-Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a wide molar range 647 anti-SNAP-25 (... Stain the Cell nuclei ( blue ) at a concentration of 1.43µM ester moiety that reacts efficiently pri-mary... Cell nuclei ( blue ) at a concentration of 1.43µM Usage Each lot of this detects... To exhibit the same species cross-reactivity as the unconjugated LC3B ( E5Q2K ) mAb... And 100 % of the antibody was purified by affinity chromatography and conjugated with Alexa 647... ( ab237374 ) protocols you may also be used to stain the nuclei... Used for protein and antibody labeling, or nucleic acid applications with high labeling density an important in... Role in T Cell development and differentiation: Rat following antigen retrieval using Sodium Citrate H.I.E.R., tissue! Anti-Mouse Alexa 647 preadsorbed secondary antibody binds to antibodies raised in rabbit used in conjunction with biotinylated primary.. Paraffin-Embedded Human brain tissue ( TPE ) microscopy of potentially explosive deposits in plumbing purified by affinity chromatography conjugated! Of Science, Shanghai, pre-adsorbed 647 Anti-CD163 antibody [ EPR361 ] conjugated to Alexa Fluor® 647 is optimal.
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